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Among other things, he noted, this could help establish a recommended dose

Heat-induced antigen retrieval was performed at 95 C for 30 min in Tris/EDTA buffer (10 mM Tris, 1 mM EDTA, pH 9.2)

The 1 mL vials often did not have any preservative in the solution, so they were only approved for one-time use

The SSc-ILD is most likely to progress during the first 4 years of systemic disease and especially in the first 2 years and in a small subset of patients, in whom lung disease precedes the cutaneous manifestations of SSc 3

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