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J 10 (4), 390397

As a proof-of-principle, targeted amplicon sequencing across TP53 gene on plasma DNA samples collected from a limited number of localised prostate cancer patients before and after local treatment suggests detection of ctDNA before surgery is associated with poor clinical outcome (metastatic-free survival) [108]

Impact of ROS on the TME The TME is a complex ecosystem composed of tumor cells, immune cells, stromal cells, and various soluble factors, playing a central role in tumor initiation, progression, and treatment resistance [177]