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The tissues were counterstained with DAPI (4,6-diamidino-2-phenylindole) for 10 min at room temperature to label the nuclei and then mounted on slides with anti-fade medium (ProLong Gold, Thermo Fisher Scientific Inc., Rockford, IL)

The number of sessions will vary depending on the area, & density of iron staining

A target protein can be separated from other molecules via specific properties like molecular mass, charge, hydrophobicity or affinity to another molecule, whereas the latter the most efficient property is

Redox regulation of the NPR1-TGA1 system of Arabidopsis thaliana by nitric oxide

Amini N, Sarkaki A, Dianat M, Mard SA, Ahangarpour A, Badavi M