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At the lymphocyte level, it inhibited the upregulation of the costimulatory molecules CD86 and CD54 on the cell surface

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Then the collected cells were treated with different initial pH (2.5, 3.0, 4.0, 5.0), various bile concentration (0.3%2%, w/v) or various sodium chloride (NaCl) concentration (2.0%8.0%, w/v) for 3 h, the survival rates were measured after treatments