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from 100 M stock in DMSO) in ERB buffer for 30 min at 37 C in ambient CO 2

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Once inside the cell, cystine is reduced to cysteine by the enzyme thioredoxin reductase 1 (TXNRD1) ( Figure 1 ) ( C - , which operates as a heterodimeric complex of SLC7A11 and SLC3A2, enables the exchange of cystine and glutamate across the plasma membrane ( Figure 1 ) ( Ferroptosis inhibitory protein 1 (FSP1) serves as an important antioxidant involved in the mechanism of ferroptosis ( Figure 1 ) ( CoQ10 can be synthesized by the mevalonate (MVA) pathway using acetyl coenzyme A ( Tetrahydrobiopterin (BH4) possesses robust antioxidant properties, enabling it to directly curb lipid peroxidation (69, Figure 1 )

For miRNA expression analysis, 200 ng total RNA was taken for polyA tail addition followed by cDNA synthesis using Quanta Biosciences 95107-025 qScript miRNA cDNA Synthesis Kit according to the manufacturers instructions (Mestdagh et al, 2014)