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After a 2h incubation at RT, wells were washed 5 times with PBS-T

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Electrophiles are electron seekers and thus accept a lone pair of electrons from a nucleophile

Regarding the stability of archaeal SAHH tetramers, the shortened C-terminal domain was speculated to either be useful for intramolecular motion at high temperature and prevent denaturation based on a protein model for Pfu SAHH 36 , or to positively affect the thermostability of the enzyme 27