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The resulting PCR products (10 L) were digested with 0.2 L of Dpn I for 2 h at 37 C to remove the methylated templates

Heart failure therapy by GLP-1RAs Although a meta-analysis of large-scale clinical trials suggested that GLP-1RAs reduce heart failure hospitalizations (HHF) by approximately 10% in patients with diabetes at high cardiovascular risk [22, 23], the treatment effects on the risk of HHF in individual trials other than AMPLITUDE-O were not significant (Tables 1, 2)

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