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Examination of the GEO dataset of estrogen-stimulated MCF-7 cells revealed that the expression of SLC7A11 and SLC3A2 , components of the system x c responsible for cystine uptake, was upregulated following estrogen treatment (Fig

Nat Rev Microbiol 2021;19(12):774-785

Here, we focused primarily on the acute mitochondrial impact of 5FU, and although we arrived at the combination of 5FU and mitochondrial inhibitors (more specifically, 5FU and metformin) somewhat serendipitously, we have now generated evidence to support the use of 5FU and metformin combinations not only in patients with resistant or relapsed disease as previously suggested by others, but also in treatment-naive, first-line chemotherapy settings

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Prior to mass spectrometry, we recommend that you elute the sample from the Dynabeads magnetic beads and then further purify it by gel electrophoresis and band excision