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Description

Lorenz RC, Gleich T, Buchert R, Schlagenhauf F, Khn S, Gallinat J

The supernatant was then collected and protein was assayed on 96-well plates by adding 3 L of standard and 150 L of protein assay dye reagent (Bio-Rad, Hercules, CA, USA) diluted 1:5 with distilled water

mTOR phosphorylation at Ser2448 indicates downstream target engagement, while S6K1 and 4E-BP1 phosphorylation reflects mTORC1 complex activity

In this review, we aim to provide a comprehensive overview of the renal actions of IGF-1 signaling, highlighting its role in the pathophysiological complications associated with chronic kidney disease (CKD) at systemic level

While these results are suggestive of ferroptosis in this pathological scenario, further work using ferroptosis-specific inhibitors such as Fer-1 will be helpful to confirm this point