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Conclusion: We estimate between 1 in 3 and 1 in 2 adults have steatosis based on two validated NITs, and 388,800-567,000 Canadians could have advanced liver fibrosis

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Heat-killed OP50-1 was produced by heating an overnight culture to 60 C for 30 min, prior to plate seeding

Bacteroidetes and some Negativicutes bacteria such as Veillonella , Dialister , synthesize propionate through succinate pathway (Anachad et al., 2023), and some Negativicutes bacteria via acrylate pathway, like Megasphaera elsdenii and Lachnospiraceae (Schoenfeld and Wojtczak, 2016), to generate propionate