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Cells were resuspended in transformation solution by vortexing (260 L 50% w/v PEG-3350, 36 L 1 M lithium acetate, 50 L 2 mg/mL ssDNA, 14 L of DNA of interest)

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Blood-brain barrier dysfunction precedes cognitive decline and neurodegeneration in diabetic insulin resistant mouse model: an implication for causal link