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Cholesterol accumulation can also impair the function of innate immune system cells, such as natural killer (NK) cellscytotoxic lymphocytes of the innate immune system that recognize and eliminate stressed, transformed, or infected cells without prior sensitizationby reducing their cytotoxic capacity and increasing the risk of HCC in the context of MASLD [35, 36]

10.1111/j.1474-9726.2008.00407.x Aging Cell 18 CambronneX

Nuclei were pelleted by centrifugation (650 g, 5 min), supernatant was discarded and the pellet was resuspended in 100 l NUN1 buffer containing 20 mM Tris-HCL (pH = 7.5), 75 mM NaCl, 0.5 mM EGTA, 50 % glycerol, 1x protease and 1x phosphatase inhibitors

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Under normal conditions, its lifespan ranges from a few microseconds to a few milliseconds (Glin 2020