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Additionally, high concentrations of estradiol stimulate anti-inflammatory cytokines such as IL-4, IL-10, and TGF-

Sigma Aldrich) in a high temperature vapour bath was performed and the endogenous peroxidase activity was quenched with 3% H 2 O 2 (VWR International, USA) in methanol (Carl Roth) for 10 min at room temperature (RT)