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Each lysate was prewarmed in a 37 C heat block for 5 min before 103 l prewarmed (37 C) reagent mixture was added such that each final reaction had 0.1 M KPO 4 (pH 7), 0.5 mM NADPH (Cayman, 9000743), 20 nmol acetyl-CoA (Cayman, 16160), 12 mM DTT (Millipore, 3483-12-3), 20 nmol [12]C-malonyl CoA (Cayman, 16455), 12 mM EDTA and 0.1 Ci 14 C-malonyl CoA (American Radiolabeled Chemicals, ARC 0755)

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