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Precursor and fragment detection were performed using an Orbitrap at a resolution MS1 = 120,000

This theory is further supported by the presence of Lewy bodies in the ENS even before their appearance in other areas of the body, including the CNS [117]

Moreover, to overcome the obstacle of its latency, the enzyme was activated with different SDS concentrations which resulted in highest levels of activity at 2 mM SDS