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DNA Cell Biol (2017) 36(4):24955

Each freeze-thaw cycle causes ice crystal formation that can denature the peptide structure

doi: 10.3390/foods12142714 5

Major efforts have been made in recent years to improve methods of decellularization, allowing for the preservation of the native ECM composition as well as the appropriate three-dimensional architecture and region-specific cues required for further cellular adhesion and differentiation 9