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Relative activity was defined as the ratio of firefly luciferase activity to Renilla luciferase activity and was calculated by dividing the luminescence intensity obtained with the assay for firefly luciferase by that of the Renilla luciferase

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10.1016/s0021-9258(20)80512-2 64 LeeM.JoA.LeeS.KimJ

This gives you the confidence that your results are based on the actual compound you intended to study