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The oxygen level dose-dependently regulates the level of HIF-1, which gradually increases following the decline of oxygen content in the air from 20 to 5% being particularly pronounced below 5% O 2 (Jiang et al., 1996)

Assessment of neurological function Global neurological deficits were quantified using the Bederson score (Supplementary Table 10)

Pegvisomant increases the metabolic rate of food-deprived mice To assess whether the energy-conserving effects of GH can be pharmacologically manipulated, we tested in mice the effects of a clinically available GHR antagonist (pegvisomant)