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After 30 min of blocking with blocking buffer (Dulbeccos PBS containing 0.2% bovine serum albumin, pH 7.4), at RT, the plates were washed 3x with wash buffer (Dulbeccos PBS containing 0.05% Tween 20, pH 7.4)

Antioxidant defense systems can be broadly categorized into four types: preventive antioxidants, radical scavenging antioxidants, repair antioxidant systems, and cellular signalling antioxidant defense systems

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Targeting mitochondrial oxidative stress with MitoQ reduces NET formation and kidney disease in lupus-prone MRL- lpr mice