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Crosslinking density and polymer chemical structures can modulate the stiffness of the final nanocarrier affecting the BBB crossing efficiency and intracellular accumulation of the cargo: as an example, Zheng et al., 2021) demonstrated that lower elasticity of PEG-block-poly(pentafluorophenyl methacrylate) NPs led to increased transcytosis and brain accumulation, despite shorter blood circulation times

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The specificity of the assay is controlled by GR

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