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Over time, however, symptoms are likely to become more noticeable

10.1093/jxb/erq432 35 FanH

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Overall, these results point to a mechanism whereby baseline reductions in mitochondrial complex I activity in PD contribute to excessive inflammation through upregulation of inflammasome activity

Materials and methods Cell culture Human acute monocytic leukemia cells, THP1, and the mouse leukemic monocyte cell line, RAW264.7, were cultured using RPMI-1640 medium modified to exclude calcium nitrate and supplemented with 2.05 mM L-Glutamine (Gibco, C11875500BT), 10% fetal bovine serum (FBS) (ExCell, FSP500), and a 1% penicillinstreptomycin mixed solution (Abiowell, AWH0529a)