bpc-157 intervertebral disc healing study Frontiers Stable Gastric Pentadecapeptide BPC 157

Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT

Sep 30 1989;2(8666):757-61

It is not a shortcut, and results build over weeks rather than days

Freezing irreversibly damages the peptide structure of semaglutide , tirzepatide , and all other GLP-1 receptor agonists

It selectively inhibits COX-2 enzymes more than COX-1, meaning its gentler on the stomach