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Then they were sterilized by adding 70% ethanol and exposing it to ultraviolet light for 30 min, followed by washing with PBS three times [34]

Physical examination revealed 4/5 proximal muscle weakness, Gottron papules, heliotrope rash, and multiple irregular ulcers with overlying eschars (Figure 1A)

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The resulting mixture was filtered, then the organic solution was washed with saturated sodium bicarbonate and brine, and then dried with anhydrous Na 2 SO 4