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In addition, CTN induced ferroptosis of human liver cancer HepG2 cells by significantly enhancing ROS accumulation, reducing GSH level, and downregulating the xCT and GPX4 expression, which could be inhibited by ferrostatin-1 (Fer-1, ferroptosis inhibitor) (Liu JL

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However aggregation is likely to be in part due to either primary nucleation in conditions that favor the Cu 1+ charge state such as hypoxia or acidity, or amorphous non-sheet aggregates induced by Cu 2+ (Jin et al., 2011)

Protocol completion at week 6 with reassessment