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In addition, the oxidative damage induced by excessive ROS and decreases in antioxidants, such as superoxide dismutase and glutathione peroxidase in diabetes also causes mitochondrial dysfunction (63)

The sections were deparaffinized, hydrated, antigen retrieved, and then probed with primary antibodies against KIM-1, IL-1, TNF-, F4/80, 4-HNE, GPX4, Ferritin, and p53 at the dilution of 1:200, followed by the incubation with the secondary antibodies for 60min on the second day