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They are naturally occurring toxins produced by filamentous fungi (molds)

Filtered viral supernatant was then concentrated by Lenti-X Concentrator (#631232, Takara) following the manufacturers instruction and the virus pellet was resuspended in 1/100th of the original volume using cold DMEM

Synthetic DNA fragments containing the BALF5 target gene as well as flanking genomic regions were synthesized as double stranded DNA gBlocks (IDT), and were used to generate a standard curve with known gene copy numbers ranging from 10 7 to 10 0 copies/l

The Ub monomer K48R mutation was introduced to prevent undesirable Ub chain elongation

a , e by two-tailed unpaired t -test