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After rinsing, the sections underwent incubation with a biotinylated secondary antibody for 30 min at 37C

The sources of EV are vast and varied, and almost all cell types can produce them (Figure 1)

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Briefly, cells were seeded in 100 L cell plating reagent in 96 well plates and allowed to incubate at 37 C (5% CO 2 , 95% relative humidity) for 24 h