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Description

DOI: 10.1515/CCLM.2007.341 14

marginale was used ( The first reaction mixture comprised 1.2 l dNTPs (2.5 mM), 0.15 l Taq polymerase (0.05 U), 1.5 l reaction buffer IB (1), 0.6 l of a solution containing the mixed primers (10 mM), and 10.05 l sterile ultra-pure water