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By integrating spintip-based affinity purification (FISAP) with data-independent acquisition (DIA) mass spectrometry, we developed a high-sensitivity workflow that identified 818 macrophage-secreted proteins modified by itaconation, including a subset trafficked via exosome-dependent secretion

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Asthma and COPD are prominent respiratory diseases typically characterised by persistent lung inflammation and oxidative stress

It shields cells from damage caused by oxidation and inflammation, it aids in detoxification, and it helps the immune system function at its best

The samples were then incubated with the equilibration buffer provided in the TUNEL assay kit at room temperature for 10 min, followed by incubation with the reaction mixture containing terminal deoxynucleotidyl transferase and labeled dUTP