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NRRL, Northern Regional Research Laboratory also known as the Agricultural Research Service (ARS) Culture Collection, United States

In its T-state, -nitrosyl hemoglobin has NO binding to the heme iron which causes histidine and iron to break forming a five-coordinated Hb-Fe(II)-NO complex (one bound to the NO and four bound to the heme porphyrin ring) and this species can be detected directly by electron paramagnetic resonance (EPR) technique [72]

10.1 min, 1% B

Western blotting Loading was performed at 40 g mass and proteins were separated by SDS-PAGE polyacrylamide gel electrophoresis

For comparison of more than two groups, standard one-way ANOVA was used