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For hPrdx6 protein purification, the crude protein extract was passed through the Ni-NTA agarose column pre-equilibrated with binding buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 10 mM Imidazole), followed by washing and elution using wash buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 60 mM Imidazole) and elution buffer (20 mM Tris (pH 7.0), 50 mM NaCl, 500 mM Imidazole), respectively

It engages in synthesizing selenoproteins, which are essential for antioxidant defense and regulating thyroid hormone levels

Summary Understanding and Treating Chapped Lips Chapped lips, whether they appear as deep cracks, dryness, or persistent peeling, can often improve with consistent lifestyle adjustments and proper daily lip care