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For this purpose, RAW 264.7 cells, a macrophage cell line obtained from a male mouse tumor induced with Abelson murine leukemia virus, were plated in complete DMEM (Euroclone) supplemented with 10% FBS in 48-well plates at a density of 2 10 5 cells per well

To our knowledge, this is one of the first studies in which a dynamic computational model based on detailed biochemistry has been used to describe individual patients and differentiate between them (see [39] for another example)

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PT-141 was subsequently developed as a targeted compound for sexual function research, and it went on to achieve full FDA approval under the brand name Vyleesi in 2019