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Zhu D, Zhang X, Wang F, Ye Q, Yang C, Liu D

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Cell pellets were resuspended in His lysis buffer (50 mM HEPES/NaOH pH 7.4, 200 mM NaCl, 20 mM imidazole, 5% (v/v) glycerol, 1mM TCEP) or Strep lysis buffer (50 mM Hepes/NaOH pH 7.4, 200 mM NaCl, 5%(v/v) Glycerol, 2 mM TCEP) supplemented with protease inhibitors and lysed using sonication

Most importantly, these results indicate that ghrelin has the potential to amplify dopamine signaling selectively in neurons that coexpress D1R and GHS-R

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