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For experiments, cells were cultured on round coverslips in 12-well plates and then stabilized for 30 min with Dulbecco's modified Eagle's medium supplemented with 5 mg/mL apotransferrin, 1 mg/mL bovine serum albumin, 25 mM HEPES (pH 7.4), 2 mM glutamine, 100 units/mL penicillin, and 100 mg/mL streptomycin (serum-free medium)

Keremi, B., Lohinai, Z., Komora, P., Duhaj, S., Borsi, K., Jobbagy-Ovari, G., Kallo, K., Szekely, A

Vitamin requirements for the reduction of homocysteine blood levels in healthy young women

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It is worth noting that HA exhibits favorable lubricating properties, particularly when cross-linked, as it forms a liquid matrix on the ocular surface