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mRNA library preparation was performed using Illumina Stranded mRNA Prep, Ligation (Illumina, #20040534) and TruSeq Stranded mRNA Library Prep (Illumina, #20020594) kits on the King Fisher FLEX system (Thermo Fisher Scientific, #18-5400620)

Identified cellular targets through which PKA activation produces a bronchodilator effect include: MLCK: Phosphorylation of myosin light chain kinase (MLCK) by cAMP-dependent protein kinase (PKA) decreases the affinity of the kinase for Ca2+ and calmodulin , resulting in a decrease in MLCK activity

Costa AG, Ramasawmy R, Ibiapina HN, Sampaio VS, Xbregas LA, Brasil LW, Tarrag AM, Almeida AC, Kuehn A, Vitor-Silva S, Melo GC

The specific screening procedure is summarized in Figure 1

In preclinical research models, exposure to GHK-Cu has been associated with altered gene expression patterns and modulation of signalling pathways related to extracellular matrix dynamics, oxidative stress response, and cellular turnover