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Histamine exists all around us: from the foods we eat, to animals and plants, and in our own tissue

Generation of constructs and transgenic lines The pF1 overexpression vector was modified from pSI103 (Sizova et al., 2001) and pEZ-GFP (Rasi et al., 2009) plasmids

[DOI] [PubMed] [Google Scholar] 11.Ivancich A., Jouve H

smegmatis Mutants Fail to Form SMRCs under Gentle Starvation To determine the role of Rel in mycobacterial resting cell differentiation, we introduced a TAG stop codon at the fourth triplet of the rel coding sequence via oligonucleotide-based site directed genome mutagenesis (recombineering) to block synthesis of the protein (see Material and Methods for details)

Monitor one or both periodically, based on clinical status