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Tandem affinity purification (TAP) or dual-tagging strategies are now commonly used in recombinant protein production: they offer a highly specific isolation of target proteins with minimal background and under mild conditions, and they are very useful in the study of protein interactions, allowing the separation of different mixed protein complexes (Arnau et al., 2006

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Pinto MC, Lima IV, da Costa FL, Rosa DV, Mendes-Goulart VA, Resende RR, Romano-Silva MA, de Oliveira AC, Gomez MV, Gomez RS (2015) Glycine transporters type 1 inhibitor promotes brain preconditioning against NMDA-induced excitotoxicity