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Samples were fixed in 3% paraformaldehyde and 0.1% glutaraldehyde (Electron Microscopy Sciences) in PBS (Corning) for 30 minutes at room temperature, followed by reduction with 0.1% NaBH 4 in PBS for 10 minutes and three times of washing with PBS

Important limitations of this study are that it was conducted only in a Japanese population, with a short follow-up time of 2 years and in a group of patients with very little progression of renal disease, since the drop of eGFR in the placebo group was less than 1 ml/min/year

10.1186/s13578-022-00776-9 42 HuiZ.WangS.LiJ.WangJ.ZhangZ

2004;287(4):L774-L783

An exaggerated inflammatory response is also implicated during the development of many gastrointestinal diseases and this is further exacerbated by depleted glutathione levels