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Slices were then incubated overnight at 4C on a shaker in primary antibody prepared in 1% FBS

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For vegans who do not supplement, slight differences in enterohepatic circulation may determine how long one can go before developing B12 deficiency symptoms (8)

Adipokines, which are cytokines secreted by adipose tissue, play crucial roles in regulating metabolic functions, including energy metabolism, inflammatory responses, and vascular homeostasis