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Comparative Antioxidant Profiles Property Glutathione (GSH) Ergothioneine (EGT) Redox activity Very high, dynamic Moderate, stable Chemical stability Low (constantly recycled) Very high Specific transporter No Yes (OCTN1/SLC22A4) Tissue accumulation Limited High (brain, liver, eyes) Mitochondrial protection Indirect (via GPx) Direct DNA protection Mitochondrial (primary) Nuclear and mitochondrial Intestinal degradation Partial Very low Human synthesis Yes (liver) No (dietary only) Mushrooms as the Only Known Source of Both Molecules While glutathione is also found in other foods asparagus, avocado, red meat ergothioneine is found almost exclusively in mushrooms

Membranes were then washed in TBS with 0.1% Tween 20, and incubated with the appropriate horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature