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Glycosidase treatment of mESCs protein extracts Protein extracts from Mettl9 Deg mESCs were prepared by lysing cells with 0.25 M NaCl, 0.05 M Tris-HCl pH 7.4, 1% NP40, 0.005 M EDTA, supplemented by Protease inhibitors

Glutathione crosses the blood-brain barrier in its liposomal form, where it protects neurons from free radical accumulation and supports mitochondrial function

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The target gene products were primarily found in various cellular locations, including the plasma membrane rafts, nuclear envelope, lumen of the endoplasmic reticulum, and oxidoreductase complexes

For hematoxylin-eosin (H&E) staining of tumor, mice from each group were sacrificed and perfused with cold PBS followed by 4% paraformaldehyde (PFA) at scheduled time