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These pathways are thought to be influenced by the hippocampus, amygdala and ventromedial prefrontal hypothalamus

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5 L BODIPY-GTP&SOS1&SA-Tb was added into each assay well and incubated for 3 h

The level of myelination for this particular mouse was not found to be an outlier compared to the rest of the cuprizone powder group, as it was not 1.5 interquartile ranges outside of the 1st or 3rd quartiles as per Tukeys outlier detection model