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doi: 10.1186/s40478-020-00924-7 223 DuanJCuiJYangZGuoCCaoJXiMet al

fTHF, 10-formyltetrahydrofolate

Protein Purification The thawed cell pellet was resuspended in 120 mL of buffer A [50 mM sodium phosphate (NaPi) (pH 7.5), 5% (v/v) glycerol, 200 mM sodium chloride (NaCl)], with 2 mM phenymethylsulfonylfluoride, 2 mM magnesium chloride, lysozyme (30 mg) and DNAse (10 mg) at 4 C, and disrupted by sonication (Branson Ultrasonic)

Darbandi M, Darbandi S, Agarwal A, Sengupta P, Durairajanayagam D, Henkel R, et al

Toxicity outcomes vary based on particle characteristics (type, size, and surface chemistry), exposure dose and duration, organismal factors (age, sex, species), and co-exposure to other pollutants