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The plates were then washed twice with ice-cold PBS, scraped, pelleted 250 g at 4 C for 3 min and lysed with RIPA buffer (50 mM Tris-Cl (pH = 8), 150 mM NaCl, 5 mM EDTA, 1 % NP-40, 0.5 % sodium deoxycholate, 0.1% SDS, 1x protease and 1x phosphatase inhibitors)

[DOI] [PMC free article] [PubMed] [Google Scholar] 29.Woolf K., Hahn N.L., Christensen M.M., Carlson-Phillips A., Hansen C.M

Pahal S, Pahal V, Chaudhary A (2025) From data to discovery: neuroinformatics in understanding Alzheimers disease

Lysyl oxidase, the copper-dependent enzyme that catalyzes the oxidative deamination of lysine residues in collagen and elastin (a step required for the cross-linking that gives mature collagen and elastin their mechanical properties), requires copper as a cofactor

Available evidence suggests a role for voltage-dependent anion channel (VDAC), located in the OMM, and adenine nucleotide translocase (ANT) across the IMM ( 2 + -induced swelling and MPT, although at a higher threshold, which has been interpreted as evidence against a role for ANT in MPT (Sileikyte et al., 2014)