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Routine maintenance involves plating them at approximately 20000 cells/cm, replacing medium daily or every other day, and passaging twice weekly avoiding full confluency

However, it is notoriously difficult to recapitulate these levels ex vivo, probably due to the prolonged differentiation process or absence of physiological stimuli

Regarding human cysteine bioavailability, it is thought that the bulk of absorbed amino acids come through enterocyte absorption, in the small intestine, and amino acids in the colonic lumen will be mainly used for bacteria metabolism or may be absorbed as di- or tri-peptides but very few as free amino acids

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