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This approach not only can be quite demanding and time-consuming, but also does not take advantage of variety of non-specific interactions (e.g

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Solution I (0.2% Coomassie Brilliant Blue G-250 (BioRad) in 90 % methanol) and solution II (20% acetic acid in Milli-Q water) were mixed in a 1:1 ratio and used to stain the gels for 20 min at room temperature

doi:10.1021/tx400204d Dubowski KM (1985) Absorption, distribution and elimination of alcohol: highway safety aspects

193 Additionally, caspase-3 cleaves GSDME at its linker region, releasing an N-terminal fragment that activates pyroptosis through a mechanism similar to that of the GSDMD N-terminal domain