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Rinsing of glass plates was done the next day and then inoculated with CoraLite594 or Fluor488-conjugated secondary antibodies (1:200) in the dark for 1 h, followed by labeling using Antifade Mounting Medium with DAPI for 10 min

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However, the degree of accuracy of these measurements is again checked with the help of percent Relative Standard Deviation (% RSD), which comes to about 3%