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Data were recorded using PSTrace software (v5, PalmSens)

The databases were then processed by generating all feasible tautomers for each ligand and assigning all possible protonation states at pH 7.0 2.0 by Epik, using all atom OPLS force field

It appears that brain iron deficiency can also occur independently of systemic iron availability, as many RLS sufferers have normal blood levels of markers of iron status (Ferre 2019