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GB112099, Servicebio, Wuhan, China) and PNP (1:300

More recently Yonezawa et al

Furthermore, the protein concentration in the supernatant was measured via a BCA protein assay kit (Elabscience, Wuhan, Hubei, China)

They showed that the survival fraction decreased by an increase of radiation dose (08 Gy 6MeV) in the presence of galactose-PEG-coated gold NPs was more than that of uncoated gold NPs which is mainly due to more amount of coated gold NPs uptake compared to uncoated

Step 2 Membrane Binding and Insertion: Upon binding to membrane-localised HDM-2, PNC-27 inserts into the lipid bilayer via its C-terminal transmembrane-penetrating domain, anchoring the PNC-27HDM-2 complex within the cell membrane