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Kovalzon and Strekalova, summarizing the field in 2006, were explicit: the link between DSIP and sleep had never been further characterized, in part because of the lack of isolation of the DSIP gene, protein and possible related receptor, and the hypothesis of DSIP as a sleep factor was extremely poorly documented and still weak (Kovalzon & Strekalova, 2006)

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Over a period of 10 years, efforts were made to delineate the enhancer core elements of the LCR [129], [130], with the aim of decreasing the size of the DNA fragments used, such that the chimeric DNA constructs would be compatible with transfer vectors derived from gamma-retroviruses [131], [132]